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20 pages, 3772 KiB

Open AccessArticle

Industrial Catalytic Production Process of Erythromycin

by Theodora Adamantidi, Ellie Panoutsopoulou, Evangelia Stavrakoudi, Panagiota Tzevelekou and Nikolaos C. Kokkinos

Processes 2024, 12(7), 1533; https://doi.org/10.3390/pr12071533 (registering DOI) - 21 Jul 2024

Abstract

The impact of COVID-19’s unexpected outbreak forced the scientific community to seek alternative treatment methods in order to overcome the hindrance of traditional medicine in terms of alleviating the symptoms of this virus. Erythromycin, which was introduced in 1952, is an antibiotic that [...] Read more.

The impact of COVID-19’s unexpected outbreak forced the scientific community to seek alternative treatment methods in order to overcome the hindrance of traditional medicine in terms of alleviating the symptoms of this virus. Erythromycin, which was introduced in 1952, is an antibiotic that is reported to pose as an effective substitute medication for various ailments such as skin, respiratory, bone, and female reproductive conditions, and cancer, as well as the newly added COVID-19. The importance of both the erythromycin molecule and the catalyst of its production, namely P450eryF of the cytochrome P450 family, in many health-concerned and environmentally related applications, has led several countries, the World Health Organization (WHO) and the health industry to recruit and cooperate with numerous universities and institutions, in an attempt to tackle the demand for efficient antibiotics. The aim of this study is to discuss and further analyze the overall structure and catalytic mechanism of erythromycin’s synthesis and industrial production, in order to gain a better comprehension of this molecule’s significance and value in the pharmaceutical field. This was carried out through the citation of the current production rates per country and the latest statistics and published patents. As implied in this manuscript, the demand for an increase and improvement in the production of erythromycin and its antibiotic derivatives should be globally promoted to deliver more effective results against infectious diseases, such as COVID-19. Full article

(This article belongs to the Special Issue Monitoring and Control of Processes in the Context of Industry 4.0)

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13 pages, 9740 KiB

Open AccessArticle

VP0 Myristoylation Is Essential for Senecavirus A Replication

by Peiyu Xiao, Liang Meng, Xingyang Cui, Xinran Liu, Lei Qin, Fandan Meng, Xuehui Cai, Dongni Kong, Tongqing An and Haiwei Wang

Pathogens 2024, 13(7), 601; https://doi.org/10.3390/pathogens13070601 (registering DOI) - 21 Jul 2024

Abstract

Many picornaviruses require the myristoylation of capsid proteins for viral replication. Myristoylation is a site-specific lipidation to the N-terminal G residue of viral proteins, which is catalyzed by the ubiquitous eukaryotic enzyme N-myristoyltransferase (NMT) by allocating the myristoyl group to the N-terminal G [...] Read more.

Many picornaviruses require the myristoylation of capsid proteins for viral replication. Myristoylation is a site-specific lipidation to the N-terminal G residue of viral proteins, which is catalyzed by the ubiquitous eukaryotic enzyme N-myristoyltransferase (NMT) by allocating the myristoyl group to the N-terminal G residue. IMP-1088 and DDD85646 are two inhibitors that can deprive NMT biological functions. Whether Senecavirus A (SVA) uses NMT to modify VP0 and regulate viral replication remains unclear. Here, we found that NMT inhibitors could inhibit SVA replication. NMT1 knock-out in BHK-21 cells significantly suppressed viral replication. In contrast, the overexpression of NMT1 in BHK-21 cells benefited viral replication. These results indicated that VP0 is a potential NMT1 substrate. Moreover, we found that the myristoylation of SVA VP0 was correlated to the subcellular distribution of this protein in the cytoplasm. Further, we evaluated which residues at the N-terminus of VP0 are essential for viral replication. The substitution of N-terminal G residue, the myristoylation site of VP0, produced a nonviable virus. The T residue at the fifth position of the substrates facilitates the binding of the substrates to NMT. And our results showed that the T residue at the fifth position of VP0 played a positive role in SVA replication. Taken together, we demonstrated that SVA VP0 myristoylation plays an essential role in SVA replication. Full article

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11 pages, 1542 KiB

Open AccessArticle

The Kinetics of Inflammation-Related Proteins and Cytokines in Children Undergoing CAR-T Cell Therapy—Are They Biomarkers of Therapy-Related Toxicities?

by Paweł Marschollek, Karolina Liszka, Monika Mielcarek-Siedziuk, Iwona Dachowska-Kałwak, Natalia Haze, Anna Panasiuk, Igor Olejnik, Tomasz Jarmoliński, Jowita Frączkiewicz, Zuzanna Gamrot, Anna Radajewska, Iwona Bil-Lula and Krzysztof Kałwak

Biomedicines 2024, 12(7), 1622; https://doi.org/10.3390/biomedicines12071622 (registering DOI) - 21 Jul 2024

Abstract

CD19-targeted CAR-T cell therapy has revolutionized the treatment of relapsed/refractory (r/r) pre-B acute lymphoblastic leukemia (ALL). However, it can be associated with acute toxicities related to immune activation, particularly cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). Cytokines released from [...] Read more.

CD19-targeted CAR-T cell therapy has revolutionized the treatment of relapsed/refractory (r/r) pre-B acute lymphoblastic leukemia (ALL). However, it can be associated with acute toxicities related to immune activation, particularly cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). Cytokines released from activated immune cells play a key role in their pathophysiology. This study was a prospective analysis of proinflammatory proteins and cytokines in children treated with tisagenlecleucel. Serial measurements of C-reactive protein, fibrinogen, ferritin, IL-6, IL-8, IL-10, IFNγ, and TNFα were taken before treatment and on consecutive days after infusion. The incidence of CRS was 77.8%, and the incidence of ICANS was 11.1%. No CRS of grade ≥ 3 was observed. All complications occurred within 14 days following infusion. Higher biomarker concentrations were found in children with CRS grade ≥ 2. Their levels were correlated with disease burden and CAR-T cell dose. While cytokine release syndrome was common, most cases were mild, primarily due to low disease burden before lymphodepleting chemotherapy (LDC). ICANS occurred less frequently but exhibited various clinical courses. None of the toxicities were fatal. All of the analyzed biomarkers rose within 14 days after CAR-T infusion, with most reaching their maximum around the third day following the procedure. Full article

(This article belongs to the Special Issue Advances in CAR-T Cell Therapy)

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9 pages, 1869 KiB

Open AccessArticle

Rare Variants of the SMN1 Gene Detected during Neonatal Screening

by Maria Akhkiamova, Aleksander Polyakov, Andrey Marakhonov, Sergey Voronin, Elena Saifullina, Zulfiia Vafina, Kristina Michalchuk, Svetlana Braslavskaya, Alena Chukhrova, Nina Ryadninskaya, Sergey Kutsev and Olga Shchagina

Genes 2024, 15(7), 956; https://doi.org/10.3390/genes15070956 (registering DOI) - 21 Jul 2024

Abstract

During the expanded neonatal screening program conducted in 2023, we analyzed samples obtained from 1,227,130 out of 1,256,187 newborns in the Russian Federation in order to detect 5q spinal muscular atrophy (5q SMA). Within the 253-sample risk group formed based on the results [...] Read more.

During the expanded neonatal screening program conducted in 2023, we analyzed samples obtained from 1,227,130 out of 1,256,187 newborns in the Russian Federation in order to detect 5q spinal muscular atrophy (5q SMA). Within the 253-sample risk group formed based on the results of the first screening stage, 5 samples showed a discrepancy between the examination results obtained via various screening methods and quantitative MLPA (used as reference). The discrepancy between the results was caused by the presence of either a c.835-18C>T intronic variant or a c.842G>C p.(Arg281Thr) missense variant in the SMN1 gene, both of which are located in the region complementary to the sequences of annealing probes for ligation and real-time PCR. Three newborns had the c.835-18C>T variant in a compound heterozygous state with a deletion of exons 7–8 of the SMN1 gene, one newborn with two copies of the SMN1 gene had the same variant in a heterozygous state, and one newborn had both variants—c.835-18C>T and c.842G>C p.(Arg281Thr)—in a compound heterozygous state. Additional examination was carried out for these variants, involving segregation analysis in families, carriage analysis in population cohorts, and RNA analysis. Based on the obtained results, according to the ACMG criteria, the c.835-18C>T intronic variant should be classified as likely benign, and the c.842G>C p.(Arg281Thr) missense substitution as a variant of uncertain clinical significance. All five probands are under dynamic monitoring. No 5q SMA symptoms were detected in these newborns neonatally or during a 1-year follow-up period. Full article

(This article belongs to the Topic Advances in Genetics and Precision Medicine in Human Diseases)

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11 pages, 445 KiB

Open AccessArticle

Evaluating Large Language Models’ Ability Using a Psychiatric Screening Tool Based on Metaphor and Sarcasm Scenarios

by Hiromu Yakura

J. Intell. 2024, 12(7), 70; https://doi.org/10.3390/jintelligence12070070 (registering DOI) - 21 Jul 2024

Abstract

Metaphors and sarcasm are precious fruits of our highly evolved social communication skills. However, children with the condition then known as Asperger syndrome are known to have difficulties in comprehending sarcasm, even if they possess adequate verbal IQs for understanding metaphors. Accordingly, researchers [...] Read more.

Metaphors and sarcasm are precious fruits of our highly evolved social communication skills. However, children with the condition then known as Asperger syndrome are known to have difficulties in comprehending sarcasm, even if they possess adequate verbal IQs for understanding metaphors. Accordingly, researchers had employed a screening test that assesses metaphor and sarcasm comprehension to distinguish Asperger syndrome from other conditions with similar external behaviors (e.g., attention-deficit/hyperactivity disorder). This study employs a standardized test to evaluate recent large language models’ (LLMs) understanding of nuanced human communication. The results indicate improved metaphor comprehension with increased model parameters; however, no similar improvement was observed for sarcasm comprehension. Considering that a human’s ability to grasp sarcasm has been associated with the amygdala, a pivotal cerebral region for emotional learning, a distinctive strategy for training LLMs would be imperative to imbue them with the ability in a cognitively grounded manner. Full article

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18 pages, 1847 KiB

Open AccessArticle

Maximizing Participation in Olfactory Training in a Sample with Post-COVID-19 Olfactory Loss

by Alice Helena Delgado-Lima, Jaime Bouhaben and María Luisa Delgado-Losada

Brain Sci. 2024, 14(7), 730; https://doi.org/10.3390/brainsci14070730 (registering DOI) - 21 Jul 2024

Abstract

Purpose: This study aims to highlight the feasibility of an olfactory training program entirely monitored through online media in COVID-19 patients. Methods: Classic olfactory training was performed with a sample with olfactory loss due COVID-19 (n = 11). Participants were engaged on [...] Read more.

Purpose: This study aims to highlight the feasibility of an olfactory training program entirely monitored through online media in COVID-19 patients. Methods: Classic olfactory training was performed with a sample with olfactory loss due COVID-19 (n = 11). Participants were engaged on a weekly video call in order to improve adherence and collect information regarding the number of correct answers and the individuals’ perception of olfactory function. The olfactory status after training was compared to two groups, one composed of participants who contracted COVID-19 but did not report olfactory loss (n = 11) and a sample composed of healthy participants (n = 11). Results: The experimental group showed improvements throughout the training period (TDI score on week 0 was 20.3 (5.6) and 24.6 (4.3) for week 12, and on week 24 was 25.4 (6.2) (F = 5.115, df = 2, 20, p = 0.016), and post hoc tests showed that participants significantly improved their TDI score in W12 compared to W0 (SMD = 0.869, p = 0.041) and in W24 compared to W0 (SMD = 0.859, p = 0.041). The experimental group showed lower scores when compared with both groups, and the no OT COVID-19 group showed lower scores than the healthy control group, even though they did not report olfactory alterations. Conclusions: Findings suggest that the strategies applied to improve adherence were successful since 100% of the sample completed the training adherence, offering a valuable framework for future olfactory training studies. Full article

(This article belongs to the Section Sensory and Motor Neuroscience)

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10 pages, 840 KiB

Open AccessReview

Are Colpodella Species Pathogenic? Nutrient Uptake and Approaches to Diagnose Infections

by Mahdi I. Salti and Tobili Y. Sam-Yellowe

Pathogens 2024, 13(7), 600; https://doi.org/10.3390/pathogens13070600 (registering DOI) - 21 Jul 2024

Abstract

Colpodella species are free-living protists phylogenetically related to apicomplexans. Colpodella sp. have been detected in human and animal tissues, as well as in ticks and biting flies. The trophozoite and cyst stages of Colpodella species can be distinguished from stages of the prey [...] Read more.

Colpodella species are free-living protists phylogenetically related to apicomplexans. Colpodella sp. have been detected in human and animal tissues, as well as in ticks and biting flies. The trophozoite and cyst stages of Colpodella species can be distinguished from stages of the prey Parabodo caudatus using Sam-Yellowe’s trichrome staining. Colpodella species obtain nutrients by attaching to their prey, aspirating the prey’s cytoplasmic contents into a posterior food vacuole and encysting. It is unclear whether both trophozoite and cyst stages are present in human and animal tissues. Molecular techniques have detected Colpodella species in human blood, cerebrospinal fluid, and in ticks and flies. However, no morphological information was reported to aid life-cycle stage identification of Colpodella species. This review discusses the increased reports of Colpodella species detection in animals and in arthropods and the need to identify stages present in human and animal tissues. We previously used Sam-Yellowe’s trichrome staining to identify life-cycle stages of Colpodella sp. In this review, we examine the reports of Colpodella species detection in human and animal tissues to determine whether the identification of Colpodella species represents true infections or contaminations of samples collected during routine surveillance of piroplasm infections in animals and arthropods. This review also aims to provide insights regarding Colpodella, nutrient uptake, and the survival of Colpodella sp. within humans, animals, and arthropods, as well as whether the attachment of trophozoites to cells occurs in tissues leading to myzocytosis and endocytosis. Full article

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13 pages, 3125 KiB

Open AccessArticle

Optically Transparent Frequency Selective Surfaces with Wideband Capability for IoT Applications: A Polarization-Independent Double-Layer Design

by Omer Faruk Gunaydin and Sultan Can

Sensors 2024, 24(14), 4724; https://doi.org/10.3390/s24144724 (registering DOI) - 21 Jul 2024

Abstract

This study proposes wide-band frequency selective surfaces (FSS) with polarization-independent characteristics that are tailored for IoT applications. The design consists of two different layers with band-stop characteristics that target key frequency bands in sub-6 GHz: 3.7 GHz (n77) and 4.5 GHz (n79), offering [...] Read more.

This study proposes wide-band frequency selective surfaces (FSS) with polarization-independent characteristics that are tailored for IoT applications. The design consists of two different layers with band-stop characteristics that target key frequency bands in sub-6 GHz: 3.7 GHz (n77) and 4.5 GHz (n79), offering a 1.39 GHz bandwidth spanning from 3.61 GHz to 5.0 GHz. This study also presents a double-layer structure with a WB property with a fractional bandwidth of 32%. Simulations have been conducted to observe variations in insertion loss across incident and polarization angles ranging from 0 to 60 degrees for both TE and TM modes in the suggested FSS structures. These simulations demonstrate the design’s polarization independence. Transparent polyvinyl chloride with a dielectric constant of 2.77 and a thickness of 1.48 mm has been utilized as the substrate material. The optical transmittance is calculated to be 96.7% for Layer 1, 95.7% for Layer 2, and 92.4% for the double-layer structure, and these calculated optical transmittance values were found to be higher compared to the studies in the literature. The proposed design is well-suited for sub-6 GHz IoT applications due to their high transparency, cost-effectiveness, robust high-performance capabilities in suppression, and polarization-independent features. The results of 3D full-wave simulations were compared with measurement and the equivalent circuit model outcomes, and a good agreement between the results was observed. Full article

(This article belongs to the Section Internet of Things)

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18 pages, 3364 KiB

Open AccessArticle

Superior Anticancer and Antifungal Activities of New Sulfanyl-Substituted Niclosamide Derivatives

by Jingyi Ma, Dileepkumar Veeragoni, Hindole Ghosh, Nicole Mutter, Gisele Barbosa, Lauren Webster, Rainer Schobert, Wendy van de Sande, Prasad Dandawate and Bernhard Biersack

Biomedicines 2024, 12(7), 1621; https://doi.org/10.3390/biomedicines12071621 (registering DOI) - 21 Jul 2024

Abstract

The approved anthelmintic salicylanilide drug niclosamide has shown promising anticancer and antimicrobial activities. In this study, new niclosamide derivatives with trifluoromethyl, trifluoromethylsulfanyl, and pentafluorosulfanyl substituents replacing the nitro group of niclosamide were prepared (including the ethanolamine salts of two promising salicylanilides) and tested [...] Read more.

The approved anthelmintic salicylanilide drug niclosamide has shown promising anticancer and antimicrobial activities. In this study, new niclosamide derivatives with trifluoromethyl, trifluoromethylsulfanyl, and pentafluorosulfanyl substituents replacing the nitro group of niclosamide were prepared (including the ethanolamine salts of two promising salicylanilides) and tested for their anticancer activities against esophageal adenocarcinoma (EAC) cells. In addition, antifungal activity against a panel of Madurella mycetomatis strains, the most abundant causative agent of the neglected tropical disease eumycetoma, was evaluated. The new compounds revealed higher activities against EAC and fungal cells than the parent compound niclosamide. The ethanolamine salt 3a was the most active compound against EAC cells (IC₅₀ = 0.8–1.0 µM), and its anticancer effects were mediated by the downregulation of anti-apoptotic proteins (BCL2 and MCL1) and by decreasing levels of β-catenin and the phosphorylation of STAT3. The plausibility of binding to the latter factors was confirmed by molecular docking. The compounds 2a and 2b showed high in vitro antifungal activity against M. mycetomatis (IC₅₀ = 0.2–0.3 µM) and were not toxic to Galleria mellonella larvae. Slight improvements in the survival rate of G. mellonella larvae infected with M. mycetomatis were observed. Thus, salicylanilides such as 2a and 3a can become new anticancer and antifungal drugs. Full article

(This article belongs to the Special Issue Small Molecule Drug Discovery with Anti-microbial and Anti-cancer Properties)

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11 pages, 1022 KiB

Open AccessArticle

Development Modeling of Phormia regina (Diptera: Calliphoridae)

by Amanda Roe, Jeffrey D. Wells and Leon G. Higley

Insects 2024, 15(7), 550; https://doi.org/10.3390/insects15070550 (registering DOI) - 21 Jul 2024

Abstract

A series of experiments were conducted on Phormia regina, a forensically important blow fly species, that met the requirements needed to create statistically valid development models. Experiments were conducted over 11 temperatures (7.5 to 32.5 °C, at 2.5 °C intervals) with a [...] Read more.

A series of experiments were conducted on Phormia regina, a forensically important blow fly species, that met the requirements needed to create statistically valid development models. Experiments were conducted over 11 temperatures (7.5 to 32.5 °C, at 2.5 °C intervals) with a 16:8 L:D cycle. Experimental units contained 20 eggs, 10 g of beef liver, and 2.5 cm of sand. Each life stage (egg to adult) had five sampling times. Each sampling time was replicated four times for a total of 20 measurements per life stage. For each sampling time, the cups were pulled from the chambers, and the stage of each maggot was documented morphologically through posterior spiracular slits and cephalopharyngeal development. Data were normally distributed with the later larval (L3m) and pupation stages having the most variation within and transitioning between stages, particularly between 12.5 °C and 20.0 °C. The biological minimum was between 10.0 °C and 12.5 °C, with little egg development and no egg emergence at 7.5 °C and no maturation past L1 at 10.0 °C. Phormia regina did not display increased mortality associated with the upper temperature of 32.5 °C. The development data generated illustrate the advantages of large data sets in modeling blow fly development and the need for curvilinear models in describing development at environmental temperatures near the biological minima and maxima. Full article

(This article belongs to the Collection Advances in Diptera Biology)

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16 pages, 2468 KiB

Open AccessArticle

Optimization of Enzymolysis Modification Conditions of Dietary Fiber from Bayberry Pomace and Its Structural Characteristics and Physicochemical and Functional Properties

by Zhaolin Zhang, Qin Ruan, Xiaoming Sun and Jianfeng Yuan

Molecules 2024, 29(14), 3415; https://doi.org/10.3390/molecules29143415 (registering DOI) - 21 Jul 2024

Abstract

Bayberry pomace, a nutrient-rich material abundant in dietary fiber (DF), has historically been underutilized due to a lack of thorough research. This study aimed to investigate the physicochemical and functional properties of the DF. Ultrasonic enzymatic treatment was performed to extract the total [...] Read more.

Bayberry pomace, a nutrient-rich material abundant in dietary fiber (DF), has historically been underutilized due to a lack of thorough research. This study aimed to investigate the physicochemical and functional properties of the DF. Ultrasonic enzymatic treatment was performed to extract the total DF, which was then optimized to produce modified soluble dietary fiber (MSDF) and insoluble dietary fiber (MIDF). The optimized conditions yielded 15.14% of MSDF with a water-holding capacity (WHC) of 54.13 g/g. The DFs were evaluated for their structural, physicochemical, and functional properties. The MSDF showed a higher (p < 0.05) WHC, oil-holding capacity (OHC), swelling capacity (SC), cation exchange capacity (CEC), and glucose adsorption capacity (GAC) (about 14.15, 0.88, 1.23, 1.22, and 0.34 times) compared to the DF. Additionally, the MSDF showed strong, superior radical scavenging and blood sugar-lowering capabilities, with a more porous surface morphology. A Fourier-transform infrared (FT-IR) spectroscopy analysis indicated that enzymatic modification degraded the cellulose and hemicellulose, reducing the DF crystallinity. Overall, the results demonstrated that cellulase hydrolysis could effectively improve the physicochemical and functional properties of DF, thereby paving the way for its development into functional food products. Full article

(This article belongs to the Special Issue Food Polysaccharides: Structure, Properties and Application II)

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19 pages, 4499 KiB

Open AccessArticle

Phosphorus-Solubilizing Bacteria Enhance Cadmium Immobilization and Gene Expression in Wheat Roots to Reduce Cadmium Uptake

by Delong Kan, Minyu Tian, Ying Ruan and Hui Han

Plants 2024, 13(14), 1989; https://doi.org/10.3390/plants13141989 (registering DOI) - 21 Jul 2024

Abstract

The application of phosphorus-solubilizing bacteria is an effective method for increasing the available phosphorus content and inhibiting wheat uptake of heavy metals. However, further research is needed on the mechanism by which phosphorus-solubilizing bacteria inhibit cadmium (Cd) uptake in wheat roots and its [...] Read more.

The application of phosphorus-solubilizing bacteria is an effective method for increasing the available phosphorus content and inhibiting wheat uptake of heavy metals. However, further research is needed on the mechanism by which phosphorus-solubilizing bacteria inhibit cadmium (Cd) uptake in wheat roots and its impact on the expression of root-related genes. Here, the effects of strain Klebsiella aerogenes M2 on Cd absorption in wheat and the expression of root-related Cd detoxification and immobilization genes were determined. Compared with the control, strain M2 reduced (64.1–64.6%) Cd uptake by wheat roots. Cd fluorescence staining revealed that strain M2 blocked the entry of exogenous Cd into the root interior and enhanced the immobilization of Cd by cell walls. Forty-seven genes related to Cd detoxification, including genes encoding peroxidase, chalcone synthase, and naringenin 3-dioxygenase, were upregulated in the Cd+M2 treatment. Strain M2 enhanced the Cd resistance and detoxification activity of wheat roots through the regulation of flavonoid biosynthesis and antioxidant enzyme activity. Moreover, strain M2 regulated the expression of genes related to phenylalanine metabolism and the MAPK signaling pathway to enhance Cd immobilization in roots. These results provide a theoretical basis for the use of phosphorus-solubilizing bacteria to remediate Cd-contaminated fields and reduce Cd uptake in wheat. Full article

(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)

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17 pages, 8480 KiB

Open AccessArticle

Characteristics of Atmospheric Diabatic Heating of the Southwest China Vortex That Induces Extreme Rainstorms in Sichuan

by Chunhua Zhou and Yueqing Li

Atmosphere 2024, 15(7), 861; https://doi.org/10.3390/atmos15070861 (registering DOI) - 21 Jul 2024

Abstract

In this study, we aimed to demonstrate the importance of diabatic heating in extreme rainstorm weather events induced by the Southwest China vortex (SWCV) in different precipitation regions with a similar circulation background. The results showed that atmospheric diabatic heating had indicative significance [...] Read more.

In this study, we aimed to demonstrate the importance of diabatic heating in extreme rainstorm weather events induced by the Southwest China vortex (SWCV) in different precipitation regions with a similar circulation background. The results showed that atmospheric diabatic heating had indicative significance for the intensity evolution of the SWCV and the precipitation area. Changes in the diabatic heating intensity preceded the intensity evolution of the SWCV, and the diabatic heating region was consistent with the heavy precipitation region. The variation in diabatic heating was mainly due to the positive contribution of its vertical transport term. The two types of spatially non-uniform heating effects were similar; however, the western type was located southeast of the SWCV, with an asymmetric distribution on the southeastern and northwestern sides. The eastern type was located in the northeast of the SWCV, with an asymmetric distribution on the northeastern and southwestern sides. The vertically non-uniform heating effect played a decisive role in the distribution and evolution of the spatially non-uniform heating terms. The vertically non-uniform heating effect affected the intensity evolution of the SWCV. In contrast, the horizontally non-uniform heating effect, in opposition to the vertically non-uniform heating effect, had a slightly weaker intensity than the vertically non-uniform heating effect. For the SWCV system, which induces extreme rainstorms, the magnitude of the horizontally non-uniform heating effect could reach that of vertically non-uniform heating; thus, the possible impact of horizontally non-uniform heating should be considered. Full article

(This article belongs to the Special Issue Advances in Rainfall-Induced Hazard Research)

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11 pages, 996 KiB

Open AccessArticle

Impact of Lactic Acid Fermentation on the Organic Acids and Sugars of Developed Oat and Buckwheat Beverages

by Kübra Küçükgöz, Anna Franczak, Wiszko Borysewicz, Klaudia Kamińska, Muhammad Salman, Wioletta Mosiej, Marcin Kruk, Danuta Kołożyn-Krajewska and Monika Trząskowska

Fermentation 2024, 10(7), 373; https://doi.org/10.3390/fermentation10070373 (registering DOI) - 21 Jul 2024

Abstract

In recent years, new plant-based foods and drinks have been developed to meet the growing demand for animal-derived alternatives, particularly dairy products. This study investigates the impact of lactic acid fermentation on the organic acids and sugars in oat and buckwheat beverages developed [...] Read more.

In recent years, new plant-based foods and drinks have been developed to meet the growing demand for animal-derived alternatives, particularly dairy products. This study investigates the impact of lactic acid fermentation on the organic acids and sugars in oat and buckwheat beverages developed using Lactobacillus johnsonii K4 and Lacticaseibacillus rhamnosus K3, which are potential probiotics. The fermented samples were analyzed for pH changes, bacterial viability, and the concentration of organic acids and sugars over 15 days. The results indicated significant variations in bacterial colony counts, with L. johnsonii K4 showing the highest initial growth. Over 15 days, pH levels decreased, with the most acidic conditions observed in buckwheat beverages. Notably, fermentation led to a significant increase in acetic acid concentration and a reduction in malic acid levels, particularly in buckwheat samples. These findings highlight the dynamic nature of fermentation in enhancing the nutritional profile and shelf-life of plant-based beverages. Full article

(This article belongs to the Special Issue Fermented Beverages Revisited: From Terroir to Customized Functional Products, 2nd Edition)

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15 pages, 3026 KiB

Open AccessSystematic Review

Is the Timing of Surgery a Sufficient Predictive Factor for Outcomes in Patients with Proximal Femur Fractures? A Systematic Review

by Mihai Rădulescu, Bogdan-Radu Necula, Sandu Aurel Mironescu, Mihai Dan Roman, Alexander Schuh and Radu-Dan Necula

J. Pers. Med. 2024, 14(7), 773; https://doi.org/10.3390/jpm14070773 (registering DOI) - 21 Jul 2024

Abstract

(1) Background: Hip fractures are currently recognized as major public health problems, raising many issues in terms of both patients’ quality of life and the cost associated with caring for this type of fracture. Many authors debate whether to operate as soon as [...] Read more.

(1) Background: Hip fractures are currently recognized as major public health problems, raising many issues in terms of both patients’ quality of life and the cost associated with caring for this type of fracture. Many authors debate whether to operate as soon as possible or to postpone surgery until the patient is stable. The purpose of this review was to review the literature and obtain additional information about the moment of surgery, the time to surgery, length of hospital stay, and how all of these factors influence patient mortality and complications. (2) Methods: The systematic search was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and PICO guidelines, using the Google Scholar platform, for articles published between 2015 and 2023. Quality assessment was performed. (3) Results: After applying the inclusion criteria, 20 articles were included in the final list. Those who had surgery within 48 h had lower in-hospital and 30-day mortality rates than those who operated within 24 h. The American Society of Anesthesiologists (ASA) score is an important predictive factor for surgical delay, length of hospital stay (LOS), complications, and mortality. (4) Conclusions: Performing surgery in the first 48 h after admission is beneficial to patients after medical stabilization. Avoidance of delayed surgery will improve postoperative complications, LOS, and mortality. Full article

(This article belongs to the Special Issue Personalized Medicine in Orthopedics and Traumatology)

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12 pages, 1270 KiB

Open AccessArticle

Enhancing Bioactivity through the Transfer of the 2-(Hydroxymethoxy)Vinyl Moiety: Application in the Modification of Tyrosol and Hinokitiol

by Marcin Molski

Molecules 2024, 29(14), 3414; https://doi.org/10.3390/molecules29143414 (registering DOI) - 21 Jul 2024

Abstract

Utilizing Density Functional Theory (DFT) calculations at the B3LYP/QZVP level and incorporating the Conductor-like Polarizable Continuum Model (C-PCM) for solvation, the thermodynamic and chemical activity properties of 21-(hydroxymethoxy)henicosadecaenal, identified in cultured freshwater pearls from the mollusk Hyriopsis cumingii, have been elucidated. The [...] Read more.

Utilizing Density Functional Theory (DFT) calculations at the B3LYP/QZVP level and incorporating the Conductor-like Polarizable Continuum Model (C-PCM) for solvation, the thermodynamic and chemical activity properties of 21-(hydroxymethoxy)henicosadecaenal, identified in cultured freshwater pearls from the mollusk Hyriopsis cumingii, have been elucidated. The study demonstrates that this compound releases formaldehyde, a potent antimicrobial agent, through dehydrogenation and deprotonation processes in both hydrophilic and lipophilic environments. Moreover, this polyenal exhibits strong anti-reductant properties, effectively scavenging free radicals. These critical properties classify the pearl-derived ingredient as a natural multi-functional compound, serving as a coloring, antiradical, and antimicrobial agent. The 2-(hydroxymethoxy)vinyl (HMV) moiety responsible for the formaldehyde release can be transferred to other compounds, thereby enhancing their biological activity. For instance, tyrosol (4-(2-hydroxyethyl)phenol) can be modified by substituting the less active 2-hydroxyethyl group with the active HMV one, and hinokitiol (4-isopropylotropolone) can be functionalized by attaching this moiety to the tropolone ring. A new type of meso-carrier, structurally modeled on pearls, with active substances loaded both in the layers and the mineral part, has been proposed. Full article

(This article belongs to the Special Issue Feature Papers in Computational and Theoretical Chemistry)

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16 pages, 8131 KiB

Open AccessArticle

Forest Structure Regulates Response of Erosion-Induced Carbon Loss to Rainfall Characteristics

by Weiwei Wang, Chao Xu, Teng-Chiu Lin, Zhijie Yang, Xiaofei Liu, Decheng Xiong, Shidong Chen, Guangshui Chen and Yusheng Yang

Forests 2024, 15(7), 1269; https://doi.org/10.3390/f15071269 (registering DOI) - 21 Jul 2024

Abstract

Forestation is a common measure to control erosion-induced soil and carbon (C) loss, but the effect can vary substantially between different types of forest. Here, we measured event-based runoff, soil, dissolved organic carbon (DOC), particulate organic carbon (POC) and total C loss with [...] Read more.

Forestation is a common measure to control erosion-induced soil and carbon (C) loss, but the effect can vary substantially between different types of forest. Here, we measured event-based runoff, soil, dissolved organic carbon (DOC), particulate organic carbon (POC) and total C loss with runoff plots (20 m × 5 m) in a broad-leaved and a coniferous forest in subtropical China and explored their relationships with rainfall amount, average intensity, maximum 5-min intensity and rainfall erosivity. The broad-leaved forest had a denser canopy but sparse understory vegetation while the coniferous forest had a relatively open canopy but dense understory vegetation. The results showed that runoff, soil, DOC, POC and total C losses were all significantly higher in the broad-leaved forest than the coniferous forest despite the potentially higher canopy interception associated with the greater leaf area index of the broad-leaved forest. The mean runoff in the broad-leaved forest was 3.03 ± 0.20 m³ ha⁻¹ event⁻¹ (mean ± standard error) and 12.49 ± 0.18 m³ ha⁻¹ event⁻¹ in the coniferous forest. The mean soil, DOC, POC and total C loss (kg ha⁻¹ event⁻¹) was 1.12 ± 0.16, 0.045 ± 0.003, 0.118 ± 0.016 and 0.163 ± 0.017, respectively, in the broad-leaved forest and 0.66 ± 0.09, 0.020 ± 0.002, 0.060 ± 0.009 and 0.081 ± 0.010, respectively, in the coniferous forest. Runoff and DOC losses were driven by rainfall in two forests, but the key rainfall characteristic driving soil, POC and total C losses was different in the broad-leaved forest from that in the coniferous forest due to their different understory patterns. Soil, POC and total C losses were mostly driven by rainfall amount in the broad-leaved forest but by EI₃₀ in the conifer forest. Our findings highlight that the response of erosion-induced carbon loss to rainfall characteristics differs between different forest types of the same age but contrasting overstory and understory vegetation covers. Moreover, our study underscores the overlooked significance of understory vegetation in regulating these effects. Thus, we call for the inclusion of understory vegetation in the modeling of soil and carbon erosion in forest ecosystems. Full article

(This article belongs to the Section Forest Ecology and Management)

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15 pages, 884 KiB

Open AccessArticle

Small for Gestational Age Calves: Part I—Concept and Definition, Contributing Prenatal Factors and Neonatal Body Morphometrics in Holstein Friesian Calves

by Maya Meesters, Mieke Van Eetvelde, Karel Verdru, Jan Govaere and Geert Opsomer

Animals 2024, 14(14), 2125; https://doi.org/10.3390/ani14142125 (registering DOI) - 21 Jul 2024

Abstract

Low birth weight (BW) calves exhibit higher mortality rates, reduced body weights at parturition, lower first-lactation milk yields, and longer parturition to first insemination intervals. In human medicine, small for gestational age (SGA) births are associated with increased perinatal morbidity and long-term metabolic [...] Read more.

Low birth weight (BW) calves exhibit higher mortality rates, reduced body weights at parturition, lower first-lactation milk yields, and longer parturition to first insemination intervals. In human medicine, small for gestational age (SGA) births are associated with increased perinatal morbidity and long-term metabolic risks. This study aimed to define SGA in Holstein Friesian (HF) calves, evaluate their body measurements and proportions, and identify its prenatal risk factors. Four linear regression models were built with weight as a function for gestation length for bull and heifer calves born from nulli- or multiparous dams. Calves with a BW below the 10th percentile were classified as SGA. Differences in body measurements were analyzed using ANOVA, and logistic regression models identified prenatal risk factors to be born SGA. Gestation length, calf sex, and dam parity were crucial variables in defining SGA. SGA calves had significantly smaller body measurements (p < 0.001) and larger body proportions (p < 0.001) compared to average and large calves. For nulliparous dams, a higher 2nd trimester temperature–humidity index (p = 0.032) and older age at parturition (>26 months, p = 0.026) significantly increased the birth of SGA calves. For multiparous dams, both low (<5800 kg, p = 0.049) and high (6700–8600 kg, p = 0.027) milk yields during gestation lead to more SGA births, although very high-yielding dams (>8600 kg) did not birth more SGA calves. This study establishes SGA in HF calves, suggests SGA calves are asymmetrical with evidence of “brain sparing”, and highlights the impact of prenatal factors on calf size at birth. Further research is needed to determine the long-term effects of being born SGA on growth, reproductive performance, and productivity. Full article

(This article belongs to the Special Issue Effects of Environmental Factors on Animal Performance: A Focus on the Occurrence of Genotype–Environment Interaction and Epigenetic Effects)

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14 pages, 2542 KiB

Open AccessArticle

Hydroxyapatite Reinforced Magnesium Alloy Composites Using the Ultrasonic-Assisted Rheo-Squeeze Casting Technique: Microstructural and Mechanical Performance Evaluation for Bone Fixture Applications

by Arunkumar Thirugnanasambandam, Ashokkumar Mohankumar, Rajendra Prasad Reddy Botta, William Woei Fong Chong, Geethapriyan Thangamani and Manoj Gupta

Crystals 2024, 14(7), 667; https://doi.org/10.3390/cryst14070667 (registering DOI) - 21 Jul 2024

Abstract

Magnesium-based biomaterials have recently been in the research spotlight in the field of biomedical engineering owing to their properties, such as density and biocompatibility that closely align with those of human bone. However, poor strength and rapid degradation impede their application as bone [...] Read more.

Magnesium-based biomaterials have recently been in the research spotlight in the field of biomedical engineering owing to their properties, such as density and biocompatibility that closely align with those of human bone. However, poor strength and rapid degradation impede their application as bone support fixtures. The present research aims to tailor the properties of Mg by using a novel ultrasonic-assisted rheo-squeeze casting approach. To satisfy the demand, pure Mg (Mg), MHA (Mg/5%HA), MZHA (Mg-1%Zn/5%HA/), and MSHA (Mg-1%Sn/5%HA) were fabricated, and various mechanical tests were conducted to assess the composite’s mechanical properties, including its microhardness, tensile strength, compressive strength, flexural strength, and impact strength. The microstructural and fractured morphology of the composites was examined by scanning electron microscopy (SEM), whereas their elemental composition was analyzed by field emission scanning electron microscopy (FESEM) equipped with elemental mapping. Comparing the MZHA, MHA, and pure Mg samples, the mechanical behavior of MSHA is significantly superior. This is due to composites containing Sn that possess finer-grained materials, which act as barriers to dislocation motion while increasing the strength of the materials. From the observed results, there is a significant improvement in the microhardness of MSHA of 64.5% when compared to that of pure Mg, and 42.7% compared to MHA. Furthermore, MSHA composites possess noticeable enhancements in tensile and compression performance of 80.8% and 58.3%, respectively, and 19% and 22.4% compared to MHA. Additionally, the impact and flexural performance of MSHA composites exhibit higher performance (41% and 42%) than pure Mg and 8% and 7% against the MHA composite. Full article

(This article belongs to the Special Issue Corrosion and Mechanical Performance of Magnesium Alloys)

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15 pages, 275 KiB

Open AccessArticle

Effectiveness of Hydrated Sodium Calcium Aluminosilicates and Discarded Date Pits as Dietary Adsorbents for Aflatoxin B1 in Enhancing Broiler Chicken Productive Performance, Hepatic Function, and Intestinal Health

by Ala E. Abudabos, Riyadh S. Aljumaah, Abdulaziz A. Alabdullatif, Ali R. Al Sulaiman, Zafar Hakmi and Abdulrahman S. Alharthi

Animals 2024, 14(14), 2124; https://doi.org/10.3390/ani14142124 (registering DOI) - 21 Jul 2024

Abstract

The research aimed to evaluate how effective hydrated sodium calcium aluminosilicates (HSCASs) and discarded date pits (DDPs) are as dietary adsorbents for aflatoxin B1 (AFB1) in enhancing the performance and health of broiler chickens aged 16 to 30 days. A total of 240 [...] Read more.

The research aimed to evaluate how effective hydrated sodium calcium aluminosilicates (HSCASs) and discarded date pits (DDPs) are as dietary adsorbents for aflatoxin B1 (AFB1) in enhancing the performance and health of broiler chickens aged 16 to 30 days. A total of 240 Ross 308 straight-run broilers were randomly allocated into four dietary groups, each with 10 replicates: a control diet, a control diet with 1000 ppb AFB1, an AFB1-contaminated diet with 0.5% HSCAS, and an AFB1-contaminated diet with 4% DDP. Incorporating HSCASs or DDPs into the AFB1-contaminated diet resulted in significant improvements across various parameters, involving increased body weight, improved feed conversion ratio, higher dressing percentage, decreased relative weights of kidney and spleen, elevated serum levels of total protein, globulin, and glucose, reduced serum alanine aminotransferase activity, and heightened hepatic protein concentration and glutathione peroxidase activity, along with diminished hepatic malondialdehyde content and glutamic oxaloacetic transaminase activity. Moreover, both supplements led to increased ileal villus height and surface area, enhanced apparent nitrogen-corrected metabolizable energy digestibility, and decreased AFB1 residues in the liver and kidney. Moreover, the dietary inclusion of DDPs significantly decreased relative liver weight, raised serum albumin concentration, lowered serum alkaline phosphatase activity, enhanced hepatic total antioxidant capacity level, and augmented ileal villus width. Conversely, the dietary addition of HSCASs significantly heightened apparent crude protein digestibility. In conclusion, the inclusion of HSCASs and DDPs in AFB1-contaminated diets can mitigate the toxic effects of AFB1 on broiler chickens, with DDPs exhibiting additional advantages in optimizing liver function and gut morphology. Full article

(This article belongs to the Collection Poultry Feeding and Gut Health)

15 pages, 1138 KiB

Open AccessReview

Revealing the Potency of Growth Factors in Bovine Colostrum

by Yalçın Mert Yalçıntaş, Hatice Duman, Jose M. Miranda López, Alicia C. Mondragón Portocarrero, Mauro Lombardo, Farid Khallouki, Wojciech Koch, Matteo Bordiga, Hesham El-Seedi, António Raposo, Jose Luiz de Brito Alves and Sercan Karav

Nutrients 2024, 16(14), 2359; https://doi.org/10.3390/nu16142359 (registering DOI) - 21 Jul 2024

Abstract

Colostrum is a nutritious milk synthesized by mammals during the postpartum period, and its rich bioactive components has led to a global increase in the consumption of bovine colostrum as a supplement. Bovine colostrum contains key components such as immunoglobulins, oligosaccharides, lactoferrin and [...] Read more.

Colostrum is a nutritious milk synthesized by mammals during the postpartum period, and its rich bioactive components has led to a global increase in the consumption of bovine colostrum as a supplement. Bovine colostrum contains key components such as immunoglobulins, oligosaccharides, lactoferrin and lysozyme. It is a special supplement source due to its natural, high bioavailability and high concentrations of growth factors. Growth factors are critical to many physiological functions, and considering its presence in the colostrum, further research must be conducted on its safe application in many bodily disorders. Growth factors contribute to wound healing, muscle and bone development, and supporting growth in children. Additionally, the molecular mechanisms have been explored, highlighting the growth factors roles in cell proliferation, tissue regeneration, and the regulation of immune responses. These findings are crucial for understanding the potential health effects of bovine colostrum, ensuring its safe use, and forming a basis for future clinical applications. This review article examines the growth factors concentration in bovine colostrum, their benefits, clinical studies, and molecular mechanisms. Full article

(This article belongs to the Special Issue Why Animal-Derived Foods Have Been, Are and Will Be Essential for Human Nutrition)

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12 pages, 237 KiB

Open AccessFeature PaperArticle

Attachment as the Catalyst for the Attribution of Complex Cognition and Emotion to Companion Cats

by Jennifer Vonk and Esther M. C. Bouma

Animals 2024, 14(14), 2123; https://doi.org/10.3390/ani14142123 (registering DOI) - 21 Jul 2024

Abstract

Companion cat caregivers ascribe complex emotions and cognitions to their cats, and these attributions are greater with a stronger attachment to their animals. We compared attributions of emotional and cognitive complexity to cats in cat caregivers and non-caregivers. We measured attitudes toward animals, [...] Read more.

Companion cat caregivers ascribe complex emotions and cognitions to their cats, and these attributions are greater with a stronger attachment to their animals. We compared attributions of emotional and cognitive complexity to cats in cat caregivers and non-caregivers. We measured attitudes toward animals, belief in animal minds, caregivers’ strength of attachment, and attachment style with a particular companion cat in 448 university students, of whom 251 had owned a cat. We asked the extent to which respondents thought that cats were capable of expressing primary and secondary emotions and cognitive capabilities. Caregivers significantly differed from non-caregivers only in the attribution of primary emotions. Belief in animal minds predicted the attribution of secondary emotions and cognition. For caregivers only, avoidant attachment style was negatively associated with the attribution of primary emotions, whereas attachment strength was positively associated with the attribution of secondary emotions and cognition. These abilities may have greater discriminatory power as most respondents ascribe primary emotions to cats, perhaps for anthropomorphic reasons. Housing conditions (indoor and outdoor) were not associated with attributions, suggesting that bonds are more important than time spent together within the home in predicting the attribution of emotional and cognitive complexity in cats. Full article

(This article belongs to the Special Issue The Complexity of the Human–Companion Animal Bond)

13 pages, 4766 KiB

Open AccessArticle

Identification and Characterization of an Alphacoronavirus in Rhinolophus sinicus and a Betacoronavirus in Apodemus ilex in Yunnan, China

by Qian Liu, Dan-Shu Wang, Zhong-Hao Lian, Jie Fang, Pei-Yu Han, Ye Qiu, Jun-Ying Zhao, Li-Dong Zong, Yun-Zhi Zhang and Xing-Yi Ge

Microorganisms 2024, 12(7), 1490; https://doi.org/10.3390/microorganisms12071490 (registering DOI) - 21 Jul 2024

Abstract

Coronaviruses (CoVs), the largest positive-sense RNA viruses, have caused infections in both humans and animals. The cross-species transmission of CoVs poses a serious threat to public health. Rodents and bats, the two largest orders of mammals, serve as significant natural reservoirs for CoVs. [...] Read more.

Coronaviruses (CoVs), the largest positive-sense RNA viruses, have caused infections in both humans and animals. The cross-species transmission of CoVs poses a serious threat to public health. Rodents and bats, the two largest orders of mammals, serve as significant natural reservoirs for CoVs. It is important to monitor the CoVs carried by bats and rodents. In this study, we collected 410 fecal samples from bats and 74 intestinal samples from rats in Yunnan Province, China. Using RT-PCR, we identified one positive sample for alphacoronavirus (TC-14) from Rhinolophus sinicus (Chinese rufous horseshoe bat) and two positive samples for betacoronavirus (GS-53, GS-56) from Apodemus ilex (Rodentia: Muridae). We successfully characterized the complete genomes of TC-14 and GS-56. Phylogenetic analysis revealed that TC-14 clustered with bat CoV HKU2 and SADS-CoV, while GS-56 was closely related to rat CoV HKU24. The identification of positive selection sites and estimation of divergence dates further helped characterize the genetic evolution of TC-14 and GS-56. In summary, this research reveals the genetic evolution characteristics of TC-14 and GS-56, providing valuable references for the study of CoVs carried by bats and rodents in Yunnan Province. Full article

(This article belongs to the Special Issue Pathogen Infection in Wildlife 2.0)

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